bleed-through / crosstalk / crossover przenik / przesłuch
| Creator: | |
| Language pair: | inglés al polaco |
| Discipline: | Medicina: Farmacia |
| Definition / notes: | If significant overlap of the excitation or emission spectra of multiple fluorophores occurs it becomes difficult to distinguish between the different signals. Consider a combination of the three fluorophores Alexa 488, Alexa 568 and Alexa 647 (see Fig. 1). It is difficult to separate emission signals from these dyes due to their strong spectral overlap, which results in signals from multiple dyes in each channel. This phenomenon is termed crosstalk, or bleed-through. Interpreting multicolor images can be challenging in this case because they arise from a mixture of signals from multiple dyes. https://www.vilber.com/spectral-unmixing/ Bleed-through (often termed crossover or crosstalk) of fluorescence emission, due to the very broad bandwidths and asymmetrical spectral profiles exhibited by many of the common fluorophores, is a fundamental problem that must be addressed in both widefield and laser scanning confocal fluorescence microscopy. The phenomenon is usually manifested by the emission of one fluorophore being detected in the photomultiplier channel or through the filter combination reserved for a second fluorophore. Bleed-through artifacts often complicate the interpretation of experimental results, particularly if subcellular co-localization of fluorophores is under investigation or quantitative measurements are necessary, such as in resonance energy transfer (FRET) and photobleaching (FRAP) studies. https://www.olympus-lifescience.com/en/microscope-resource/primer/techniques/confocal/bleedthrough/ Spectral crosstalk includes both excitation crosstalk (cross-excitation), where multiple fluorophores are excited with a single excitation wavelength, and emission bleed-through, where unwanted fluorescent signal from a dye with overlapping emission (and excitation) spectrum is detected in the emission window. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703511/ Przesłuch – zakłócenie występujące przy przesyłaniu sygnałów, nie tylko fonicznych, od których pochodzi jego nazwa (np. w telefonii, radiofonii), rodzaj przeniku. https://pl.wikipedia.org/wiki/Przes%C5%82uch przenik, telekom. zakłócenie sygnału polegające na przenikaniu do jego drogi przesyłowej niepożądanej energii z dróg przesyłowych innych sygnałów; w telefonii zw. przesłuchem, w telewizji — prześwitem. https://encyklopedia.pwn.pl/haslo/przenik;3963513.html FM 1–43FX ma szersze spektrum emisji niż CM – DiI i SP – DiI, co doprowadziło do przesłuchu (przenikania) podczas uzyskiwania obrazu wielokanałowego za pomocą DAPI i GFP. To przeciekanie wymagało przetwarzania obrazu w celu odjęcia oddzielnych sygnałów fluorescencyjnych (rysunek uzupełniający 3). https://pol.legatechnics.com/clarity-compatible-lipophilic-dyes-31284058 |
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