This question was closed without grading. Reason: Other
Jan 19, 2022 17:46
2 yrs ago
36 viewers *
French term
prêt à l’emploi au 1/3
French to English
Medical
Biology (-tech,-chem,micro-)
Febrile neutropenia -
Here is the context:
LEICA-Bond Polymer Detection on deparaffinised sections, after antigen restoration by heat
Antibodies
Kappa (Dako polyclonal, dilution 1/2000), Lambda (Dako polyclonal, prêt à l’emploi au 1/3)
Result: the plasma cell infiltrate shows positive for the kappa and lambda chains, demonstrating its polytypical nature.
Thank you!
LEICA-Bond Polymer Detection on deparaffinised sections, after antigen restoration by heat
Antibodies
Kappa (Dako polyclonal, dilution 1/2000), Lambda (Dako polyclonal, prêt à l’emploi au 1/3)
Result: the plasma cell infiltrate shows positive for the kappa and lambda chains, demonstrating its polytypical nature.
Thank you!
Proposed translations
(English)
| 3 | ready-to-use, 1/3 (pre)diluted |
Bourth
|
Change log
Jan 19, 2022 18:24: Yolanda Broad changed "Term asked" from " prêt à l’emploi au 1/3 (GFM-Ideal study)" to " prêt à l’emploi au 1/3"
Proposed translations
1 hr
ready-to-use, 1/3 (pre)diluted
It seems that, for Dako at least, "ready to use" means "prediluted":
"Store most prediluted (“ready to use”) antibodies, their conjugates and monoclonal antibody solutions at 2–8°C because freezing and thawing is known to have a deleterious effect on their performance"
http://www.ihcworld.com/_books/Dako_Handbook.pdf
So unless what follows is relevant somehow, I assume it is the dilution factor.
"KAPPA Light Chains C C0222 APC. Affinity-isolated F(ab')2 100 tests, 1 mL C F0434 FITC. Affinity-isolated F(ab')2 100 tests, 1 mL C R0436 RPE. Affinity-isolated F(ab')2 100 tests, 1 mL These reagents have been produced in a manner that ensures a particularly wide specificity for kappa-chains. Most B cells, with the exception of pre-B progenitors, pre-B cells and mature plasma cells, express immunoglobulin on their surface. Each cell expresses only one light chain type. In normal peripheral blood and lymph nodes there is a mixture of kappa+ and lambda+ cells with TWO-THIRDS OF THE CELLS EXPRESSING KAPPA and ONE-THIRD EXPRESSING LAMBDA (1)."
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Note added at 1 hr (2022-01-19 19:05:46 GMT)
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While nothing is impossible, presumably this does not imply further dilution of the pre-dilute, since this would go against Dako recommendations:
"Reagents have been optimally diluted. Further dilution may result in loss of antigen staining."
https://www.accessdata.fda.gov/cdrh_docs/pdf15/P150025c.pdf
"Store most prediluted (“ready to use”) antibodies, their conjugates and monoclonal antibody solutions at 2–8°C because freezing and thawing is known to have a deleterious effect on their performance"
http://www.ihcworld.com/_books/Dako_Handbook.pdf
So unless what follows is relevant somehow, I assume it is the dilution factor.
"KAPPA Light Chains C C0222 APC. Affinity-isolated F(ab')2 100 tests, 1 mL C F0434 FITC. Affinity-isolated F(ab')2 100 tests, 1 mL C R0436 RPE. Affinity-isolated F(ab')2 100 tests, 1 mL These reagents have been produced in a manner that ensures a particularly wide specificity for kappa-chains. Most B cells, with the exception of pre-B progenitors, pre-B cells and mature plasma cells, express immunoglobulin on their surface. Each cell expresses only one light chain type. In normal peripheral blood and lymph nodes there is a mixture of kappa+ and lambda+ cells with TWO-THIRDS OF THE CELLS EXPRESSING KAPPA and ONE-THIRD EXPRESSING LAMBDA (1)."
--------------------------------------------------
Note added at 1 hr (2022-01-19 19:05:46 GMT)
--------------------------------------------------
While nothing is impossible, presumably this does not imply further dilution of the pre-dilute, since this would go against Dako recommendations:
"Reagents have been optimally diluted. Further dilution may result in loss of antigen staining."
https://www.accessdata.fda.gov/cdrh_docs/pdf15/P150025c.pdf
Note from asker:
| Thank you! |
| I have had to bring this to the attention of the client, based on Sue's comments |
Peer comment(s):
| agree |
Adrian MM.
1 hr
|
| disagree |
Sue Davis
: Please see my notes in the discussion
1 day 12 hrs
|
Discussion
it also begs the question of what the starting solution was i.e. the undiluted solution. In my experience dilution always refers to dilution of the solution that is commercialised in use by the lab/ 1:50, 1:100 etc. Applying a dilution factor to an antibody before purchase makes no sense.
The concentration of this prediluted antibody is 4µg/ml."